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Sak and Sak4 recombinases are required for bacteriophage replication in Staphylococcus aureus

Overview of attention for article published in Nucleic Acids Research, May 2017
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  • In the top 5% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (92nd percentile)
  • High Attention Score compared to outputs of the same age and source (95th percentile)

Mentioned by

news
2 news outlets
twitter
22 tweeters
facebook
3 Facebook pages

Readers on

mendeley
9 Mendeley
Title
Sak and Sak4 recombinases are required for bacteriophage replication in Staphylococcus aureus
Published in
Nucleic Acids Research, May 2017
DOI 10.1093/nar/gkx308
Pubmed ID
Authors

Neamah, Maan M., Mir-Sanchis, Ignacio, López-Sanz, María, Acosta, Sonia, Baquedano, Ignacio, Haag, Andreas F., Marina, Alberto, Ayora, Silvia, Penadés, José R., Maan M. Neamah, Ignacio Mir-Sanchis, María López-Sanz, Sonia Acosta, Ignacio Baquedano, Andreas F. Haag, Alberto Marina, Silvia Ayora, José R. Penadés

Abstract

DNA-single strand annealing proteins (SSAPs) are recombinases frequently encoded in the genome of many bacteriophages. As SSAPs can promote homologous recombination among DNA substrates with an important degree of divergence, these enzymes are involved both in DNA repair and in the generation of phage mosaicisms. Here, analysing Sak and Sak4 as representatives of two different families of SSAPs present in phages infecting the clinically relevant bacterium Staphylococcus aureus, we demonstrate for the first time that these enzymes are absolutely required for phage reproduction. Deletion of the genes encoding these enzymes significantly reduced phage replication and the generation of infectious particles. Complementation studies revealed that these enzymes are required both in the donor (after prophage induction) and in the recipient strain (for infection). Moreover, our results indicated that to perform their function SSAPs require the activity of their cognate single strand binding (Ssb) proteins. Mutational studies demonstrated that the Ssb proteins are also required for phage replication, both in the donor and recipient strain. In summary, our results expand the functions attributed to the Sak and Sak4 proteins, and demonstrate that both SSAPs and Ssb proteins are essential for the life cycle of temperate staphylococcal phages.

Twitter Demographics

The data shown below were collected from the profiles of 22 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 9 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 4 44%
Student > Bachelor 2 22%
Student > Master 1 11%
Researcher 1 11%
Unspecified 1 11%
Other 0 0%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 44%
Unspecified 2 22%
Agricultural and Biological Sciences 2 22%
Immunology and Microbiology 1 11%

Attention Score in Context

This research output has an Altmetric Attention Score of 31. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 January 2018.
All research outputs
#398,654
of 11,459,905 outputs
Outputs from Nucleic Acids Research
#208
of 19,743 outputs
Outputs of similar age
#19,062
of 265,622 outputs
Outputs of similar age from Nucleic Acids Research
#15
of 317 outputs
Altmetric has tracked 11,459,905 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 96th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 19,743 research outputs from this source. They receive a mean Attention Score of 5.0. This one has done particularly well, scoring higher than 98% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 265,622 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 92% of its contemporaries.
We're also able to compare this research output to 317 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 95% of its contemporaries.