Title |
The intricate network between the p34 and p44 subunits is central to the activity of the transcription/DNA repair factor TFIIH
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Published in |
Nucleic Acids Research, August 2017
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DOI | 10.1093/nar/gkx743 |
Pubmed ID | |
Authors |
Laura Radu, Elisabeth Schoenwetter, Cathy Braun, Julien Marcoux, Wolfgang Koelmel, Dominik R. Schmitt, Jochen Kuper, Sarah Cianférani, Jean M. Egly, Arnaud Poterszman, Caroline Kisker |
Abstract |
The general transcription factor IIH (TFIIH) is a multi-protein complex and its 10 subunits are engaged in an intricate protein-protein interaction network critical for the regulation of its transcription and DNA repair activities that are so far little understood on a molecular level. In this study, we focused on the p44 and the p34 subunits, which are central for the structural integrity of core-TFIIH. We solved crystal structures of a complex formed by the p34 N-terminal vWA and p44 C-terminal zinc binding domains from Chaetomium thermophilum and from Homo sapiens. Intriguingly, our functional analyses clearly revealed the presence of a second interface located in the C-terminal zinc binding region of p34, which can rescue a disrupted interaction between the p34 vWA and the p44 RING domain. In addition, we demonstrate that the C-terminal zinc binding domain of p34 assumes a central role with respect to the stability and function of TFIIH. Our data reveal a redundant interaction network within core-TFIIH, which may serve to minimize the susceptibility to mutational impairment. This provides first insights why so far no mutations in the p34 or p44 TFIIH-core subunits have been identified that would lead to the hallmark nucleotide excision repair syndromes xeroderma pigmentosum or trichothiodystrophy. |
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