| Title |
Identification of a new ribose methylation in the 18S rRNA of S. cerevisiae
|
|---|---|
| Published in |
Nucleic Acids Research, February 2015
|
| DOI | 10.1093/nar/gkv058 |
| Pubmed ID | |
| Authors |
Jun Yang, Sunny Sharma, Peter Kötter, Karl-Dieter Entian |
| Abstract |
Methylation of ribose sugars at the 2'-OH group is one of the major chemical modifications in rRNA, and is catalyzed by snoRNA directed C/D box snoRNPs. Previous biochemical and computational analyses of the C/D box snoRNAs have identified and mapped a large number of 2'-OH ribose methylations in rRNAs. In the present study, we systematically analyzed ribose methylations of 18S rRNA in Saccharomyces cerevisiae, using mung bean nuclease protection assay and RP-HPLC. Unexpectedly, we identified a hitherto unknown ribose methylation at position G562 in the helix 18 of 5' central domain of yeast 18S rRNA. Furthermore, we identified snR40 as being responsible to guide snoRNP complex to catalyze G562 ribose methylation, which makes it only second snoRNA known so far to target three ribose methylation sites: Gm562, Gm1271 in 18S rRNA, and Um898 in 25S rRNA. Our sequence and mutational analysis of snR40 revealed that snR40 uses the same D' box and methylation guide sequence for both Gm562 and Gm1271 methylation. With the identification of Gm562 and its corresponding snoRNA, complete set of ribose methylations of 18S rRNA and their corresponding snoRNAs have finally been established opening great prospects to understand the physiological function of these modifications. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 2 | 50% |
| Unknown | 2 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 75% |
| Scientists | 1 | 25% |
Mendeley readers
The data shown below were compiled from readership statistics for 27 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Switzerland | 1 | 4% |
| Unknown | 26 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 10 | 37% |
| Researcher | 8 | 30% |
| Student > Master | 3 | 11% |
| Professor > Associate Professor | 2 | 7% |
| Student > Bachelor | 2 | 7% |
| Other | 1 | 4% |
| Unknown | 1 | 4% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 13 | 48% |
| Biochemistry, Genetics and Molecular Biology | 11 | 41% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 4% |
| Nursing and Health Professions | 1 | 4% |
| Chemistry | 1 | 4% |
| Other | 0 | 0% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 July 2015.
All research outputs
#15,982,793
of 25,373,627 outputs
Outputs from Nucleic Acids Research
#22,457
of 27,550 outputs
Outputs of similar age
#199,326
of 360,581 outputs
Outputs of similar age from Nucleic Acids Research
#139
of 248 outputs
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